منابع مشابه
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals.
Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This light-driven, four-photon reaction is catalyzed by the Mn(4)CaO(5) cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However,...
متن کاملComparison of focusing optics for femtosecond X-ray diffraction
We characterize and compare four different types of focusing optics for hard X-rays, suitable for femtosecond X-ray diffraction experiments, using a tabletop femtosecond laser-based plasma source. We demonstrate a 23μm focus with a toroidally bent Ge single crystal. A maximum flux of 7 × 108 photons/(s mm2) is generated in a 32μm focus using a multi-layer mirror. An elliptical glass capillary y...
متن کاملSerial femtosecond X-ray diffraction of enveloped virus microcrystals
Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ∼700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ∼40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks ...
متن کاملFemtosecond X-ray diffraction from two-dimensional protein crystals
X-ray diffraction patterns from two-dimensional (2-D) protein crystals obtained using femtosecond X-ray pulses from an X-ray free-electron laser (XFEL) are presented. To date, it has not been possible to acquire transmission X-ray diffraction patterns from individual 2-D protein crystals due to radiation damage. However, the intense and ultrafast pulses generated by an XFEL permit a new method ...
متن کاملDirectly observing squeezed phonon states with femtosecond x-ray diffraction.
Squeezed states are quantum states of a harmonic oscillator in which the variance of two conjugate variables each oscillate out of phase. Ultrafast optical excitation of crystals can create squeezed phonon states, where the variance of the atomic displacements oscillates due to a sudden change in the interatomic bonding strength. With femtosecond x-ray diffraction we measure squeezing oscillati...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2006
ISSN: 0108-7673
DOI: 10.1107/s010876730609996x